IPG strip,Denaturing
slab gel,Denaturing
30% acrylamide/bisacrylamide (250 ml); Tris-Cl pH 8.8 (150 ml); MilliQ (millipore) (187 ml); 10% sds (6 ml); 10% ammonium persulfate (6 ml): 10% TEMED (830 µl)
excitation: 532 nm
emission: 560 LP or 610 BP filter
exposure time: 0.800
[Protocol text in here]
[Protocol text in here]