<?xml version="1.0" encoding="ISO-8859-1"?>
<GelML xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
		xsi:schemaLocation="http://www.psidev.info/gelml/GelML-v1_1.xsd"
		xmlns:fuge="http://fuge.sourceforge.net/fuge/1.0"
		xmlns="http://www.psidev.info/gelml/1.0"
		identifier="ExPASy:GelML:155">

<cvList>
<cv fullName="PSI sepCV" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/sep.obo" id="sepCV" version="1.0"/>
<cv fullName="Unit Ontology" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/unit.obo" id="UO" version="1.2"/>
</cvList>


 
		
<Gel2DExperiment id="ex51:Gel2DExperiment155"
	name="Phakopsora pachyrhizi germinating urediniospore 2-D gel" date="2008-05-02">
	<electrophoresisType>
		<cvParam cvRef="sepCV" accession="sep:00155" name="Two-Dimensional electrophoresis"/>
	</electrophoresisType>
	<ContactRole Contact_ref="ex51:Contact22">
        <role>
		<cvParam cvRef="sepCV" accession="sep:00035" name="principle investigator"/>
		</role>
	</ContactRole>








   



   

	
<Gel2DApplication Gel2DProtocol_ref="ex51:Gel2DProtocol155" id="ex51:Gel2DAppli155">


	
	
	<inputFirstDimension>
		


<Gel id="ex51:Gel1" name="Isoelectric focusing (IEF)" separationDimension="1">
	<gelType>IPG strip,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="GE Healthcare Life Sciences"/>
		<model name="Immobiline DryStrip pH 3-10NL, 13cm">
			<userParam name="Model number" value="17-6001-14"/>
		</model>
		<userParam name="Batch number" value="unknown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="130" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="3" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="0.5" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="4%"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="37" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputFirstDimension>
	
   
	
	
	
	<inputSecondDimension>
		


<Gel id="ex51:Gel2" name="Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)" separationDimension="2">
	<gelType>slab gel,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="Bio-Rad Laboratories"/>
		<model name="Criterion XL Precast Gel ">
			<userParam name="Model number" value="345-0127"/>
		</model>
		<userParam name="Batch number" value="unknown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="140" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="100" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="1" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="4-12%"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="37" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputSecondDimension>
	
   

<output>
<Gel2D id="ex51:Gel2D155">

    
	
	
    
	<PHRange dimension="1">
		<Range>
			<lowerLimit><PropertyValue value="10"/></lowerLimit>
			<upperLimit><PropertyValue value="220"/></upperLimit>
		</Range>
		<rangeType><cvParam cvRef="sepCV" accession="" name=""/></rangeType>
	</PHRange>
	

    
	
	
    
	
	<MolWeightRange dimension="2">
        <Range>
            <lowerLimit><PropertyValue value="" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></lowerLimit>
            <upperLimit><PropertyValue value="7" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></upperLimit>
        </Range>
		<rangeType><cvParam cvRef="sepCV" accession="sep:00019" name="logarithmic distribution"/></rangeType>
	</MolWeightRange>
	

</Gel2D>
</output>
<ChildProtocolApplications>
<!-- TO BE DONE -->

</ChildProtocolApplications>
</Gel2DApplication>

	

<ImageAcquisitionApplication Image_ref="ex51:Image69" Material_ref="ex51:Gel2D155" ImageAcquisitionProtocol_ref="ex51:ImageAcquisitionProtocol12" id="ex51:ImageAcquisitionApplication84">
	
	<protocolText>Exposure time - 50ms
Wavelength - visible light</protocolText>
	
</ImageAcquisitionApplication>



	
</Gel2DExperiment>






	
		
		
	
<GelMLDataCollection>
<Image location="http://miapegeldb.expasy.org/download/121/d2eEGFQc/" id="ex51:Image69" name="050208gel" bitDepth="1-bit (monochrome)" dimensionX="1350" dimensionY="1102">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="150" unitAccession="UO:0000242" unitName="pixels per inch" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00053" name="TIFF"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>




















<GelMLProtocolCollection>

    <ElectrophoresisProtocol
name="FirstDimension"
id="ex51:ElectrophoresisProtocol180" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex51:AddBufferAction" SubstanceMixtureProtocol_ref="ex51:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    <ElectrophoresisProtocol
name="SecondDimension"
id="ex51:ElectrophoresisProtocol181" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex51:AddBufferAction" SubstanceMixtureProtocol_ref="ex51:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    
<ImageAcquisitionProtocol id="ex51:ImageAcquisitionProtocol12">
	<equipment id="ex51:GenericEquipment26" name="FluorChem">
		<userParam name="type of equipment" value="camera" cvRef="sepCV" accession="sep:00099" />
		<userParam name="make" value="Alpha Innotech"/>
		
		<userParam name="version" value="8900"/>
		
	</equipment>
	<software id="ex51:GenericSoftware65" name="FluorChem" version="8900"/>
	<AutoCalibration id="ex51:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


</GelMLProtocolCollection>

<AuditCollection>

	<Person
id="ex51:Contact22"
name="Dr. Douglas G. Luster"
email="doug.luster@ars.usda.gov"
address="USDA - ARS - FDWSRU
1301 Ditto Avenue
Ft. Detrick, MD 21702">
<affiliations
Organization_ref="ex51:Organization19"/>
</Person>

	<affiliations
Organization_ref="ex51:Organization19"
name="USDA-ARS"/>

</AuditCollection>
</GelML>



