<?xml version="1.0" encoding="ISO-8859-1"?>
<GelML xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
		xsi:schemaLocation="http://www.psidev.info/gelml/GelML-v1_1.xsd"
		xmlns:fuge="http://fuge.sourceforge.net/fuge/1.0"
		xmlns="http://www.psidev.info/gelml/1.0"
		identifier="ExPASy:GelML:118">

<cvList>
<cv fullName="PSI sepCV" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/sep.obo" id="sepCV" version="1.0"/>
<cv fullName="Unit Ontology" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/unit.obo" id="UO" version="1.2"/>
</cvList>


 
		
<Gel2DExperiment id="ex31:Gel2DExperiment118"
	name="TCA-acetone-phenol_3" date="2007-04-30">
	<electrophoresisType>
		<cvParam cvRef="sepCV" accession="sep:00155" name="Two-Dimensional electrophoresis"/>
	</electrophoresisType>
	<ContactRole Contact_ref="ex31:Contact13">
        <role>
		<cvParam cvRef="sepCV" accession="sep:00035" name="principle investigator"/>
		</role>
	</ContactRole>








   



   

	
<Gel2DApplication Gel2DProtocol_ref="ex31:Gel2DProtocol118" id="ex31:Gel2DAppli118">


	
	
	<inputFirstDimension>
		


<Gel id="ex31:Gel1" name="Isoelectric focusing (IEF)" separationDimension="1">
	<gelType>IPG strip,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="BioRad"/>
		<model name="IPG strip 17 cm pH 5-8 ">
			<userParam name="Model number" value="163-2011"/>
		</model>
		<userParam name="Batch number" value="unknown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="17.1" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
		<y value="0.33" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
		<z value="0.05" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="4 "/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="32.3" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputFirstDimension>
	
   
	
	
	
	<inputSecondDimension>
		


<Gel id="ex31:Gel2" name="Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)" separationDimension="2">
	<gelType>slab gel,Denaturing</gelType>
	
	<GelManufactureApplication>
	
		<protocolText>
		34 % of destilated water
40 % of a 30 % acrylamide/Bis solution
25 % of 1.5 M Tris-HCl pH 8.8
1 % of a 10 % SDS solution
0.5 % of a 10% APS solution
0.05% of TEMED
		</protocolText>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="20" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
		<y value="20" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
		<z value="0.1" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="12"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="37.5" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputSecondDimension>
	
   

<output>
<Gel2D id="ex31:Gel2D118">

    
	
	
    
	<PHRange dimension="1">
		<Range>
			<lowerLimit><PropertyValue value="100"/></lowerLimit>
			<upperLimit><PropertyValue value="12"/></upperLimit>
		</Range>
		<rangeType><cvParam cvRef="sepCV" accession="" name=""/></rangeType>
	</PHRange>
	

    
	
	
    
	
	<MolWeightRange dimension="2">
        <Range>
            <lowerLimit><PropertyValue value="" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></lowerLimit>
            <upperLimit><PropertyValue value="7" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></upperLimit>
        </Range>
		<rangeType><cvParam cvRef="sepCV" accession="sep:00019" name="logarithmic distribution"/></rangeType>
	</MolWeightRange>
	

</Gel2D>
</output>
<ChildProtocolApplications>
<!-- TO BE DONE -->

</ChildProtocolApplications>
</Gel2DApplication>

	

<ImageAcquisitionApplication Image_ref="ex31:Image54" Material_ref="ex31:Gel2D118" ImageAcquisitionProtocol_ref="ex31:ImageAcquisitionProtocol5" id="ex31:ImageAcquisitionApplication64">
	
	<protocolText>100x100 microns</protocolText>
	
</ImageAcquisitionApplication>



	
</Gel2DExperiment>






	
		
		
	
<GelMLDataCollection>
<Image location="http://miapegeldb.expasy.org/download/23/cKVUX36T/" id="ex31:Image54" name="TCA-acetone-phenol_3" bitDepth="8-bit (256 colors)" dimensionX="1850" dimensionY="1694">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="100" unitAccession="UO:0000239" unitName="micrometers per pixel" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00053" name="TIFF"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>




















<GelMLProtocolCollection>

    <ElectrophoresisProtocol
name="FirstDimension"
id="ex31:ElectrophoresisProtocol139" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex31:AddBufferAction" SubstanceMixtureProtocol_ref="ex31:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    <ElectrophoresisProtocol
name="SecondDimension"
id="ex31:ElectrophoresisProtocol140" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex31:AddBufferAction" SubstanceMixtureProtocol_ref="ex31:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    
<ImageAcquisitionProtocol id="ex31:ImageAcquisitionProtocol5">
	<equipment id="ex31:GenericEquipment15" name="Molecular Imager FX Pro Plus multiImager System">
		<userParam name="type of equipment" value="fluorescent scanner" cvRef="sepCV" accession="sep:00100" />
		<userParam name="make" value="BioRad"/>
		
	</equipment>
	<software id="ex31:GenericSoftware37" name="PDQuest" version="7.1"/>
	<AutoCalibration id="ex31:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


</GelMLProtocolCollection>

<AuditCollection>

	<Person
id="ex31:Contact13"
name="Prof. Jesus V. Jorrin Novo"
email="bf1jonoj@uco.es"
address="Dpto. Bioquimica y Biologia Molecular, Universidad de Cordoba, Campus de Rabanales, edificio Severo Ochoa, planta baja, 14014, Cordoba, Spain">
<affiliations
Organization_ref="ex31:Organization12"/>
</Person>

	<affiliations
Organization_ref="ex31:Organization12"
name="Agricultural and Plant Biochemistry and Proteomics Research Group, Dept. Biochemistry and Molecular Biology, University of Córdoba, Spain"/>

</AuditCollection>
</GelML>



