<?xml version="1.0" encoding="ISO-8859-1"?>
<GelML xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
		xsi:schemaLocation="http://www.psidev.info/gelml/GelML-v1_1.xsd"
		xmlns:fuge="http://fuge.sourceforge.net/fuge/1.0"
		xmlns="http://www.psidev.info/gelml/1.0"
		identifier="ExPASy:GelML:349">

<cvList>
<cv fullName="PSI sepCV" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/sep.obo" id="sepCV" version="1.0"/>
<cv fullName="Unit Ontology" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/unit.obo" id="UO" version="1.2"/>
</cvList>


 
		
<Gel2DExperiment id="ex159:Gel2DExperiment349"
	name="NCB5_vs_NCB90_repl1" date="2010-07-21">
	<electrophoresisType>
		<cvParam cvRef="sepCV" accession="sep:00180" name="Difference gel electrophoresis"/>
	</electrophoresisType>
	<ContactRole Contact_ref="ex159:Contact69">
        <role>
		<cvParam cvRef="sepCV" accession="sep:00035" name="principle investigator"/>
		</role>
	</ContactRole>








   



   

	
<Gel2DApplication Gel2DProtocol_ref="ex159:Gel2DProtocol349" id="ex159:Gel2DAppli349">


	
	
	<inputFirstDimension>
		


<Gel id="ex159:Gel1" name="Isoelectric focusing (IEF)" separationDimension="1">
	<gelType>IPG strip,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="GE Healthcare"/>
		<model name="24 cm Immobiline (TM) DryStrips NL pH 3-10">
			<userParam name="Model number" value="17-6002-44 "/>
		</model>
		<userParam name="Batch number" value="unknown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="240" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="3" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="0.5" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="4"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="32" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputFirstDimension>
	
   
	
	
	
	<inputSecondDimension>
		


<Gel id="ex159:Gel2" name="Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)" separationDimension="2">
	<gelType>slab gel,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="Gel Company"/>
		<model name="2D DALT NF 12.5% pre cast polyacrylamid gels">
			<userParam name="Model number" value="1019-21"/>
		</model>
		<userParam name="Batch number" value="unknown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="260" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="200" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="1" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="12.5"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="37.5" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputSecondDimension>
	
   

<output>
<Gel2D id="ex159:Gel2D349">

    
	
	
    
	<PHRange dimension="1">
		<Range>
			<lowerLimit><PropertyValue value="100"/></lowerLimit>
			<upperLimit><PropertyValue value="6"/></upperLimit>
		</Range>
		<rangeType><cvParam cvRef="sepCV" accession="" name=""/></rangeType>
	</PHRange>
	

    
	
	
    
	
	<MolWeightRange dimension="2">
        <Range>
            <lowerLimit><PropertyValue value="" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></lowerLimit>
            <upperLimit><PropertyValue value="7" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></upperLimit>
        </Range>
		<rangeType><cvParam cvRef="sepCV" accession="sep:00019" name="logarithmic distribution"/></rangeType>
	</MolWeightRange>
	

</Gel2D>
</output>
<ChildProtocolApplications>
<!-- TO BE DONE -->

</ChildProtocolApplications>
</Gel2DApplication>

	

<ImageAcquisitionApplication Image_ref="ex159:Image179" Material_ref="ex159:Gel2D349" ImageAcquisitionProtocol_ref="ex159:ImageAcquisitionProtocol56" id="ex159:ImageAcquisitionApplication203">
	
	<protocolText>For gels conatining Cy-labeled proteins the following excitation/emission wavelengths were used: 
Cy2 488 nm/520 nm BP40; 
Cy3 532 nm/580 nm BP 30; 
Cy5 633 nm/670 nm BP30. 

For lava purple stained gels excitation/emission wavelengths were as follows 532 nm/560 nm LP. </protocolText>
	
</ImageAcquisitionApplication>



	

<ImageAcquisitionApplication Image_ref="ex159:Image180" Material_ref="ex159:Gel2D349" ImageAcquisitionProtocol_ref="ex159:ImageAcquisitionProtocol56" id="ex159:ImageAcquisitionApplication203">
	
	<protocolText>For gels conatining Cy-labeled proteins the following excitation/emission wavelengths were used: 
Cy2 488 nm/520 nm BP40; 
Cy3 532 nm/580 nm BP 30; 
Cy5 633 nm/670 nm BP30. 

For lava purple stained gels excitation/emission wavelengths were as follows 532 nm/560 nm LP. </protocolText>
	
</ImageAcquisitionApplication>



	

<ImageAcquisitionApplication Image_ref="ex159:Image181" Material_ref="ex159:Gel2D349" ImageAcquisitionProtocol_ref="ex159:ImageAcquisitionProtocol56" id="ex159:ImageAcquisitionApplication203">
	
	<protocolText>For gels conatining Cy-labeled proteins the following excitation/emission wavelengths were used: 
Cy2 488 nm/520 nm BP40; 
Cy3 532 nm/580 nm BP 30; 
Cy5 633 nm/670 nm BP30. 

For lava purple stained gels excitation/emission wavelengths were as follows 532 nm/560 nm LP. </protocolText>
	
</ImageAcquisitionApplication>



	
</Gel2DExperiment>






	
		
		
	
<GelMLDataCollection>
<Image location="http://miapegeldb.expasy.org/download/585/QaeyBnti/" id="ex159:Image179" name="NCB5_1" bitDepth="32-bit (TrueColor)" dimensionX="2385" dimensionY="1928">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="96" unitAccession="UO:0000240" unitName="dots per inch" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00054" name="BMP"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>



	
		
		
	
<GelMLDataCollection>
<Image location="http://miapegeldb.expasy.org/download/585/QJjX2Toi/" id="ex159:Image180" name="NCB90_1" bitDepth="32-bit (TrueColor)" dimensionX="2385" dimensionY="1928">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="96" unitAccession="UO:0000240" unitName="dots per inch" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00054" name="BMP"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>



	
		
		
	
<GelMLDataCollection>
<Image location="http://miapegeldb.expasy.org/download/585/tKpVqJey/" id="ex159:Image181" name="STD10" bitDepth="32-bit (TrueColor)" dimensionX="2385" dimensionY="1928">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="96" unitAccession="UO:0000240" unitName="dots per inch" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00054" name="BMP"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>




















<GelMLProtocolCollection>

    <ElectrophoresisProtocol
name="FirstDimension"
id="ex159:ElectrophoresisProtocol422" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex159:AddBufferAction" SubstanceMixtureProtocol_ref="ex159:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    <ElectrophoresisProtocol
name="SecondDimension"
id="ex159:ElectrophoresisProtocol423" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex159:AddBufferAction" SubstanceMixtureProtocol_ref="ex159:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    
<ImageAcquisitionProtocol id="ex159:ImageAcquisitionProtocol56">
	<equipment id="ex159:GenericEquipment63" name="Typhoon 9400 Varaible Mode Imager">
		<userParam name="type of equipment" value="fluorescent scanner" cvRef="sepCV" accession="sep:00100" />
		<userParam name="make" value="GE Healthcare"/>
		
	</equipment>
	<software id="ex159:GenericSoftware189" name="Tyhoon Scanner Software" version="v5.0"/>
	<AutoCalibration id="ex159:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


    
<ImageAcquisitionProtocol id="ex159:ImageAcquisitionProtocol56">
	<equipment id="ex159:GenericEquipment63" name="Typhoon 9400 Varaible Mode Imager">
		<userParam name="type of equipment" value="fluorescent scanner" cvRef="sepCV" accession="sep:00100" />
		<userParam name="make" value="GE Healthcare"/>
		
	</equipment>
	<software id="ex159:GenericSoftware189" name="Tyhoon Scanner Software" version="v5.0"/>
	<AutoCalibration id="ex159:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


    
<ImageAcquisitionProtocol id="ex159:ImageAcquisitionProtocol56">
	<equipment id="ex159:GenericEquipment63" name="Typhoon 9400 Varaible Mode Imager">
		<userParam name="type of equipment" value="fluorescent scanner" cvRef="sepCV" accession="sep:00100" />
		<userParam name="make" value="GE Healthcare"/>
		
	</equipment>
	<software id="ex159:GenericSoftware189" name="Tyhoon Scanner Software" version="v5.0"/>
	<AutoCalibration id="ex159:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


</GelMLProtocolCollection>

<AuditCollection>

	<Person
id="ex159:Contact69"
name="Claude P. Muller"
email="claude.muller@CRP-SANTE.LU"
address="Institute of Immunology, 
Centre de Recherche Public de la Santé / National Public Health Laboratory, 
20A rue Auguste Lumière, 
L-1950 Luxembourg, 
Grand-Duchy of Luxembourg
">
<affiliations
Organization_ref="ex159:Organization56"/>
</Person>

	<affiliations
Organization_ref="ex159:Organization56"
name="Institute of Immunology, Centre de Recherche Public de la Santé / National Public Health Laboratory"/>

</AuditCollection>
</GelML>



