IPG strip,Denaturing
slab gel,Denaturing
For gels conatining Cy-labeled proteins the following excitation/emission wavelengths were used:
Cy2 488 nm/520 nm BP40;
Cy3 532 nm/580 nm BP 30;
Cy5 633 nm/670 nm BP30.
For lava purple stained gels excitation/emission wavelengths were as follows 532 nm/560 nm LP.
For gels conatining Cy-labeled proteins the following excitation/emission wavelengths were used:
Cy2 488 nm/520 nm BP40;
Cy3 532 nm/580 nm BP 30;
Cy5 633 nm/670 nm BP30.
For lava purple stained gels excitation/emission wavelengths were as follows 532 nm/560 nm LP.
For gels conatining Cy-labeled proteins the following excitation/emission wavelengths were used:
Cy2 488 nm/520 nm BP40;
Cy3 532 nm/580 nm BP 30;
Cy5 633 nm/670 nm BP30.
For lava purple stained gels excitation/emission wavelengths were as follows 532 nm/560 nm LP.
[Protocol text in here]
[Protocol text in here]