<?xml version="1.0" encoding="ISO-8859-1"?>
<GelML xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
		xsi:schemaLocation="http://www.psidev.info/gelml/GelML-v1_1.xsd"
		xmlns:fuge="http://fuge.sourceforge.net/fuge/1.0"
		xmlns="http://www.psidev.info/gelml/1.0"
		identifier="ExPASy:GelML:334">

<cvList>
<cv fullName="PSI sepCV" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/sep.obo" id="sepCV" version="1.0"/>
<cv fullName="Unit Ontology" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/unit.obo" id="UO" version="1.2"/>
</cvList>


 
		
<Gel2DExperiment id="ex157:Gel2DExperiment334"
	name="Analysis of  freezing stress modulated secretome of Hippophae rhamnoides" date="2010-04-01">
	<electrophoresisType>
		<cvParam cvRef="sepCV" accession="sep:00155" name="Two-Dimensional electrophoresis"/>
	</electrophoresisType>
	<ContactRole Contact_ref="ex157:Contact68">
        <role>
		<cvParam cvRef="sepCV" accession="sep:00035" name="principle investigator"/>
		</role>
	</ContactRole>








   



   

	
<Gel2DApplication Gel2DProtocol_ref="ex157:Gel2DProtocol334" id="ex157:Gel2DAppli334">


	
	
	<inputFirstDimension>
		


<Gel id="ex157:Gel1" name="Isoelectric focusing (IEF)" separationDimension="1">
	<gelType>IPG strip,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="G E Healthcare Life Sciences"/>
		<model name="IPG Strip 3-10 non linear">
			<userParam name="Model number" value="17-6001-14 "/>
		</model>
		<userParam name="Batch number" value="Unknown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="130" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="3" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="0.5" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="4"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="32" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputFirstDimension>
	
   
	
	
	
	<inputSecondDimension>
		


<Gel id="ex157:Gel2" name="Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)" separationDimension="2">
	<gelType>slab gel,Denaturing</gelType>
	
	<GelManufactureApplication>
	
		<protocolText>
			
			

	See reference:
    CSH Protocols (2006) doi:10.1101pdb.prot4540:doi:10.1101pdb.prot4540

	See URL: http://vetbiotech.um.ac.ir/parameters/vetbiotech/filemanager/new_admin/electrophorsis/SDS-Polyacrylamide%20Gel%20Electrophoresis%20of%20Proteins.pdf


		</protocolText>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="180" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="160" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="1" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="15 "/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="30" crossLinker="0.8">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputSecondDimension>
	
   

<output>
<Gel2D id="ex157:Gel2D334">

    
	
	
    
	<PHRange dimension="1">
		<Range>
			<lowerLimit><PropertyValue value="14.2"/></lowerLimit>
			<upperLimit><PropertyValue value="97"/></upperLimit>
		</Range>
		<rangeType><cvParam cvRef="sepCV" accession="" name=""/></rangeType>
	</PHRange>
	

    
	
	
    
	
	<MolWeightRange dimension="2">
        <Range>
            <lowerLimit><PropertyValue value="" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></lowerLimit>
            <upperLimit><PropertyValue value="7" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></upperLimit>
        </Range>
		<rangeType><cvParam cvRef="sepCV" accession="sep:00019" name="logarithmic distribution"/></rangeType>
	</MolWeightRange>
	

</Gel2D>
</output>
<ChildProtocolApplications>
<!-- TO BE DONE -->

</ChildProtocolApplications>
</Gel2DApplication>

	

<ImageAcquisitionApplication Image_ref="ex157:Image151" Material_ref="ex157:Gel2D334" ImageAcquisitionProtocol_ref="ex157:ImageAcquisitionProtocol55" id="ex157:ImageAcquisitionApplication191">
	
	<protocolText>Image was acquired 2 sec exposure time
</protocolText>
	
</ImageAcquisitionApplication>



	
</Gel2DExperiment>






	
		
	
<GelMLDataCollection>
<Image location="http://pubs.acs.org/doi/abs/10.1021/pr200944z?mi=qryllt&af=R&pageSize=20&searchText=ATP" id="ex157:Image151" name="HRHAMNOIDES_SEEDLINGS (ECP)_3-10" bitDepth="8-bit (256 colors)" dimensionX="1153" dimensionY="1000">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="102" unitAccession="UO:0000242" unitName="pixels per inch" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00053" name="TIFF"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>




















<GelMLProtocolCollection>

    <ElectrophoresisProtocol
name="FirstDimension"
id="ex157:ElectrophoresisProtocol398" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex157:AddBufferAction" SubstanceMixtureProtocol_ref="ex157:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    <ElectrophoresisProtocol
name="SecondDimension"
id="ex157:ElectrophoresisProtocol399" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex157:AddBufferAction" SubstanceMixtureProtocol_ref="ex157:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    
<ImageAcquisitionProtocol id="ex157:ImageAcquisitionProtocol55">
	<equipment id="ex157:GenericEquipment61" name="Alpha imager">
		<userParam name="type of equipment" value="camera" cvRef="sepCV" accession="sep:00099" />
		<userParam name="make" value="AlphaImager gel documentation system"/>
		
		<userParam name="version" value="AlphaImager HP System"/>
		
	</equipment>
	<software id="ex157:GenericSoftware184" name="Alpha View Software" version="Alpha View Software"/>
	<AutoCalibration id="ex157:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
		
			
		
		<userParam name="configuration file" value="http://miapegeldb.expasy.org/download/580/8sTpfnID/"/>
	
</ImageAcquisitionProtocol>


</GelMLProtocolCollection>

<AuditCollection>

	<Person
id="ex157:Contact68"
name="Dr. Renu Deswal"
email="rdeswal@botany.du.ac.in"
address="Molecular Plant Physiology and Proteomics Laboratory, Department of Botany, University of Delhi">
<affiliations
Organization_ref="ex157:Organization55"/>
</Person>

	<affiliations
Organization_ref="ex157:Organization55"
name="University of Delhi"/>

</AuditCollection>
</GelML>



