IPG strip,Denaturing slab gel,Denaturing A recipe to prepare 1200 ml of 12.5% polyacrylamide gels [150.0 g acrylamide, 4.032 g bis-acrylamide, 1.5 M Tris-HCl pH 8.8, 10% SDS (w/v), 10% APS (w/v) and 340 µl TEMED] (the stirred gel solution was degassed under vacuum for 20 minutes, and APS and TEMED were not added until gels were ready to pour) was used to make 12 gels.

The gel images were acquired in 16 bit grayscale with a resolution of 300 dpi and were saved in tiff format. No filters or image processing tools were used during acquisition. [Protocol text in here] [Protocol text in here]