<?xml version="1.0" encoding="ISO-8859-1"?>
<GelML xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
		xsi:schemaLocation="http://www.psidev.info/gelml/GelML-v1_1.xsd"
		xmlns:fuge="http://fuge.sourceforge.net/fuge/1.0"
		xmlns="http://www.psidev.info/gelml/1.0"
		identifier="ExPASy:GelML:313">

<cvList>
<cv fullName="PSI sepCV" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/sep.obo" id="sepCV" version="1.0"/>
<cv fullName="Unit Ontology" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/unit.obo" id="UO" version="1.2"/>
</cvList>


 
		
<Gel2DExperiment id="ex146:Gel2DExperiment313"
	name="Fusarium graminearum strain 453 reference map" date="2010-09-18">
	<electrophoresisType>
		<cvParam cvRef="sepCV" accession="sep:00180" name="Difference gel electrophoresis"/>
	</electrophoresisType>
	<ContactRole Contact_ref="ex146:Contact63">
        <role>
		<cvParam cvRef="sepCV" accession="sep:00035" name="principle investigator"/>
		</role>
	</ContactRole>








   



   

	
<Gel2DApplication Gel2DProtocol_ref="ex146:Gel2DProtocol313" id="ex146:Gel2DAppli313">


	
	
	<inputFirstDimension>
		


<Gel id="ex146:Gel1" name="Isoelectric focusing (IEF)" separationDimension="1">
	<gelType>IPG strip,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="BioRad"/>
		<model name="ReadyStrip IPG Strips, pH 3–10 nonlinear, 24 cm,">
			<userParam name="Model number" value="163-2043"/>
		</model>
		<userParam name="Batch number" value="unknown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="240" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="3" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="0.5" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="4"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="32.5" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputFirstDimension>
	
   
	
	
	
	<inputSecondDimension>
		


<Gel id="ex146:Gel2" name="Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)" separationDimension="2">
	<gelType>slab gel,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="Serva Electrophoresis"/>
		<model name="2D Gel DALTtwelve 12.5 % Kit">
			<userParam name="Model number" value="43319.01"/>
		</model>
		<userParam name="Batch number" value="unkown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="260" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="200" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="1" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="12.5"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="32.5" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputSecondDimension>
	
   

<output>
<Gel2D id="ex146:Gel2D313">

    
	
	
    
	<PHRange dimension="1">
		<Range>
			<lowerLimit><PropertyValue value="5"/></lowerLimit>
			<upperLimit><PropertyValue value="200"/></upperLimit>
		</Range>
		<rangeType><cvParam cvRef="sepCV" accession="" name=""/></rangeType>
	</PHRange>
	

    
	
	
    
	
	<MolWeightRange dimension="2">
        <Range>
            <lowerLimit><PropertyValue value="" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></lowerLimit>
            <upperLimit><PropertyValue value="7" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></upperLimit>
        </Range>
		<rangeType><cvParam cvRef="sepCV" accession="sep:00019" name="logarithmic distribution"/></rangeType>
	</MolWeightRange>
	

</Gel2D>
</output>
<ChildProtocolApplications>
<!-- TO BE DONE -->

</ChildProtocolApplications>
</Gel2DApplication>

	

<ImageAcquisitionApplication Image_ref="ex146:Image135" Material_ref="ex146:Gel2D313" ImageAcquisitionProtocol_ref="ex146:ImageAcquisitionProtocol51" id="ex146:ImageAcquisitionApplication177">
	
	<protocolText>1) Turn on the Typhoon and leave the instrument to
warm up for at least 30 min prior to scanning. 
2) Ensure that the gel glass plates are clean, dry and free from lint.
3)Select scan area 
4)Select the appropriate wavelenths and adjust the intensity of the lasers in a way to have the maximum signal with no saturation. The adjustemnt can be done using a scanning resolution of 1mm
5)After calibration scan the gel setting the resolution to 100 um.</protocolText>
	
</ImageAcquisitionApplication>



	

<ImageAcquisitionApplication Image_ref="ex146:Image136" Material_ref="ex146:Gel2D313" ImageAcquisitionProtocol_ref="ex146:ImageAcquisitionProtocol51" id="ex146:ImageAcquisitionApplication177">
	
	<protocolText>1) Turn on the Typhoon and leave the instrument to
warm up for at least 30 min prior to scanning. 
2) Ensure that the gel glass plates are clean, dry and free from lint.
3)Select scan area 
4)Select the appropriate wavelenths and adjust the intensity of the lasers in a way to have the maximum signal with no saturation. The adjustemnt can be done using a scanning resolution of 1mm
5)After calibration scan the gel setting the resolution to 100 um.</protocolText>
	
</ImageAcquisitionApplication>



	

<ImageAcquisitionApplication Image_ref="ex146:Image137" Material_ref="ex146:Gel2D313" ImageAcquisitionProtocol_ref="ex146:ImageAcquisitionProtocol51" id="ex146:ImageAcquisitionApplication177">
	
	<protocolText>1) Turn on the Typhoon and leave the instrument to
warm up for at least 30 min prior to scanning. 
2) Ensure that the gel glass plates are clean, dry and free from lint.
3)Select scan area 
4)Select the appropriate wavelenths and adjust the intensity of the lasers in a way to have the maximum signal with no saturation. The adjustemnt can be done using a scanning resolution of 1mm
5)After calibration scan the gel setting the resolution to 100 um.</protocolText>
	
</ImageAcquisitionApplication>



	
</Gel2DExperiment>






	
		
		
	
<GelMLDataCollection>
<Image location="http://miapegeldb.expasy.org/download/514/F6dUF4Ox/" id="ex146:Image135" name="Cy3" bitDepth="8-bit (256 colors)" dimensionX="741" dimensionY="1000">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="254" unitAccession="UO:0000242" unitName="pixels per inch" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00053" name="TIFF"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>



	
		
		
	
<GelMLDataCollection>
<Image location="http://miapegeldb.expasy.org/download/514/LUu4Z1f3/" id="ex146:Image136" name="Cy5" bitDepth="8-bit (256 colors)" dimensionX="741" dimensionY="1000">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="254" unitAccession="UO:0000242" unitName="pixels per inch" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00053" name="TIFF"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>



	
		
		
	
<GelMLDataCollection>
<Image location="http://miapegeldb.expasy.org/download/514/gejgoMF4/" id="ex146:Image137" name="Cy2" bitDepth="8-bit (256 colors)" dimensionX="741" dimensionY="1000">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="254" unitAccession="UO:0000242" unitName="pixels per inch" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00053" name="TIFF"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>




















<GelMLProtocolCollection>

    <ElectrophoresisProtocol
name="FirstDimension"
id="ex146:ElectrophoresisProtocol371" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex146:AddBufferAction" SubstanceMixtureProtocol_ref="ex146:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    <ElectrophoresisProtocol
name="SecondDimension"
id="ex146:ElectrophoresisProtocol372" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex146:AddBufferAction" SubstanceMixtureProtocol_ref="ex146:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    
<ImageAcquisitionProtocol id="ex146:ImageAcquisitionProtocol51">
	<equipment id="ex146:GenericEquipment57" name="Typhoon 9400">
		<userParam name="type of equipment" value="laser scanner" cvRef="sepCV" accession="sep:00100" />
		<userParam name="make" value="GE Healthcare"/>
		
	</equipment>
	<software id="ex146:GenericSoftware168" name="Typhoon Scanner Control" version="unknown"/>
	<AutoCalibration id="ex146:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


    
<ImageAcquisitionProtocol id="ex146:ImageAcquisitionProtocol51">
	<equipment id="ex146:GenericEquipment57" name="Typhoon 9400">
		<userParam name="type of equipment" value="laser scanner" cvRef="sepCV" accession="sep:00100" />
		<userParam name="make" value="GE Healthcare"/>
		
	</equipment>
	<software id="ex146:GenericSoftware168" name="Typhoon Scanner Control" version="unknown"/>
	<AutoCalibration id="ex146:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


    
<ImageAcquisitionProtocol id="ex146:ImageAcquisitionProtocol51">
	<equipment id="ex146:GenericEquipment57" name="Typhoon 9400">
		<userParam name="type of equipment" value="laser scanner" cvRef="sepCV" accession="sep:00100" />
		<userParam name="make" value="GE Healthcare"/>
		
	</equipment>
	<software id="ex146:GenericSoftware168" name="Typhoon Scanner Control" version="unknown"/>
	<AutoCalibration id="ex146:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


</GelMLProtocolCollection>

<AuditCollection>

	<Person
id="ex146:Contact63"
name="Dr. Tommaso Serchi"
email="serchi@lippmann.lu"
address="41, rue du Brill, L4422 Belvaux, Luxembourg">
<affiliations
Organization_ref="ex146:Organization50"/>
</Person>

	<affiliations
Organization_ref="ex146:Organization50"
name="Centre de Recherce Public Gabriel Lippmann"/>

</AuditCollection>
</GelML>



