IPG strip,Denaturing slab gel,Denaturing For each independent experiment, six gels were manufactured using the following receipe: 161.2 ml Milli-Q water, 124.8 ml 30 % acrylamide/Bis solution, 98.8 ml buffer (1.5 M Tris-HCl pH 8.8, 4% SDS), 5.8 ml 10% APS solution, 260 µl TEMED

'gel' and 'Coomassie Blue' were chosen, and image acquisition was conducted after preview scan. [Protocol text in here] [Protocol text in here]