IPG strip,Denaturing
slab gel,Denaturing
For each independent experiment, six gels were manufactured using the following receipe:
161.2 ml Milli-Q water, 124.8 ml 30 % acrylamide/Bis solution, 98.8 ml buffer (1.5 M Tris-HCl pH 8.8, 4% SDS), 5.8 ml 10% APS solution, 260 µl TEMED
'gel' and 'Coomassie Blue' were chosen, and image acquisition was conducted after preview scan.
[Protocol text in here]
[Protocol text in here]