IPG strip,Denaturing
See reference:
Poznanovic, S., G. Schwall, H. Zengerling, and M. A. Cahill. 2005. Isoelectric focusing in serial immobilized pH gradient gels to improve protein separation in proteomic analysis. Electrophoresis 26:3185–3190. (2005) 26:3185-3190
See URL: http://iai.asm.org/cgi/reprint/79/10/3872
slab gel,Denaturing
See reference:
Poznanovic, S., G. Schwall, H. Zengerling, and M. A. Cahill. 2005. Isoelectric focusing in serial immobilized pH gradient gels to improve protein separation in proteomic analysis. Electrophoresis 26:3185–3190. (2005) 26:3185-3190
See URL: http://iai.asm.org/cgi/reprint/79/10/3872
DEFAULT COMPANY SETTINGS FOR 2D PAGE IMAGING
[Protocol text in here]
[Protocol text in here]