<?xml version="1.0" encoding="ISO-8859-1"?>
<GelML xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
		xsi:schemaLocation="http://www.psidev.info/gelml/GelML-v1_1.xsd"
		xmlns:fuge="http://fuge.sourceforge.net/fuge/1.0"
		xmlns="http://www.psidev.info/gelml/1.0"
		identifier="ExPASy:GelML:297">

<cvList>
<cv fullName="PSI sepCV" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/sep.obo" id="sepCV" version="1.0"/>
<cv fullName="Unit Ontology" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/unit.obo" id="UO" version="1.2"/>
</cvList>


 
		
<Gel2DExperiment id="ex131:Gel2DExperiment297"
	name="Filifactor alocis-Total proteome" date="2011-10-10">
	<electrophoresisType>
		<cvParam cvRef="sepCV" accession="sep:00155" name="Two-Dimensional electrophoresis"/>
	</electrophoresisType>
	<ContactRole Contact_ref="ex131:Contact55">
        <role>
		<cvParam cvRef="sepCV" accession="sep:00035" name="principle investigator"/>
		</role>
	</ContactRole>








   



   

	
<Gel2DApplication Gel2DProtocol_ref="ex131:Gel2DProtocol297" id="ex131:Gel2DAppli297">


	
	
	<inputFirstDimension>
		


<Gel id="ex131:Gel1" name="Isoelectric focusing (IEF)" separationDimension="1">
	<gelType>IPG strip,Denaturing</gelType>
	
	<GelManufactureApplication>
	
		<protocolText>
			
			

	See reference:
    Poznanovic, S., G. Schwall, H. Zengerling, and M. A. Cahill. 2005. Isoelectric focusing in serial immobilized pH gradient gels to improve protein separation in proteomic analysis. Electrophoresis 26:3185–3190. (2005) 26:3185-3190

	See URL: http://iai.asm.org/cgi/reprint/79/10/3872


		</protocolText>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="7" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
		<y value="3" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
		<z value="1" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="10"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="3.2" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputFirstDimension>
	
   
	
	
	
	<inputSecondDimension>
		


<Gel id="ex131:Gel2" name="Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)" separationDimension="2">
	<gelType>slab gel,Denaturing</gelType>
	
	<GelManufactureApplication>
	
		<protocolText>
			
			

	See reference:
    Poznanovic, S., G. Schwall, H. Zengerling, and M. A. Cahill. 2005. Isoelectric focusing in serial immobilized pH gradient gels to improve protein separation in proteomic analysis. Electrophoresis 26:3185–3190. (2005) 26:3185-3190

	See URL: http://iai.asm.org/cgi/reprint/79/10/3872


		</protocolText>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="7" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
		<y value="5" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
		<z value="1" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="10%"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="3.2" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputSecondDimension>
	
   

<output>
<Gel2D id="ex131:Gel2D297">

    
	
	
    
	<PHRange dimension="1">
		<Range>
			<lowerLimit><PropertyValue value="10"/></lowerLimit>
			<upperLimit><PropertyValue value="250"/></upperLimit>
		</Range>
		<rangeType><cvParam cvRef="sepCV" accession="" name=""/></rangeType>
	</PHRange>
	

    
	
	
    
	
	<MolWeightRange dimension="2">
        <Range>
            <lowerLimit><PropertyValue value="" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></lowerLimit>
            <upperLimit><PropertyValue value="7" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></upperLimit>
        </Range>
		<rangeType><cvParam cvRef="sepCV" accession="sep:00019" name="logarithmic distribution"/></rangeType>
	</MolWeightRange>
	

</Gel2D>
</output>
<ChildProtocolApplications>
<!-- TO BE DONE -->

</ChildProtocolApplications>
</Gel2DApplication>

	

<ImageAcquisitionApplication Image_ref="ex131:Image128" Material_ref="ex131:Gel2D297" ImageAcquisitionProtocol_ref="ex131:ImageAcquisitionProtocol46" id="ex131:ImageAcquisitionApplication166">
	
	<protocolText>DEFAULT  COMPANY SETTINGS FOR 2D PAGE IMAGING </protocolText>
	
</ImageAcquisitionApplication>



	
</Gel2DExperiment>






	
		
		
	
<GelMLDataCollection>
<Image location="http://miapegeldb.expasy.org/download/427/zP9e14sH/" id="ex131:Image128" name="FA-2D" bitDepth="8-bit (256 colors)" dimensionX="1000" dimensionY="1000">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="1000" unitAccession="UO:0000242" unitName="pixels per inch" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00048" name=".psd"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>




















<GelMLProtocolCollection>

    <ElectrophoresisProtocol
name="FirstDimension"
id="ex131:ElectrophoresisProtocol348" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex131:AddBufferAction" SubstanceMixtureProtocol_ref="ex131:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    <ElectrophoresisProtocol
name="SecondDimension"
id="ex131:ElectrophoresisProtocol349" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex131:AddBufferAction" SubstanceMixtureProtocol_ref="ex131:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    
<ImageAcquisitionProtocol id="ex131:ImageAcquisitionProtocol46">
	<equipment id="ex131:GenericEquipment52" name="LAUNCH VISION WORK LS">
		<userParam name="type of equipment" value="camera" cvRef="sepCV" accession="sep:00099" />
		<userParam name="make" value="UVP"/>
		
		<userParam name="version" value="UVP"/>
		
	</equipment>
	<software id="ex131:GenericSoftware146" name="LAUNCH VISION WORKS LS" version="LAUNCH VISION WORKS LS"/>
	<AutoCalibration id="ex131:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


</GelMLProtocolCollection>

<AuditCollection>

	<Person
id="ex131:Contact55"
name="WILSON ARUNI, FRANCIS ROY and HANSEL .M.FLETCHER"
email="waruni@llu.edu"
address="DIVISION OF MICROBIOLOGY AND MOLECULAR GENETICS
SCHOOL OF MEDICINE 
LOMA LINDA UNIVERSITY
LOMA LINDA -CA-92354,USA">
<affiliations
Organization_ref="ex131:Organization44"/>
</Person>

	<affiliations
Organization_ref="ex131:Organization44"
name="FLETCHER LAB , SCHOOL OF MEDICINE ,LOMA LINDA UNIVERSITY"/>

</AuditCollection>
</GelML>



