<?xml version="1.0" encoding="ISO-8859-1"?>
<GelML xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
		xsi:schemaLocation="http://www.psidev.info/gelml/GelML-v1_1.xsd"
		xmlns:fuge="http://fuge.sourceforge.net/fuge/1.0"
		xmlns="http://www.psidev.info/gelml/1.0"
		identifier="ExPASy:GelML:292">

<cvList>
<cv fullName="PSI sepCV" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/sep.obo" id="sepCV" version="1.0"/>
<cv fullName="Unit Ontology" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/unit.obo" id="UO" version="1.2"/>
</cvList>


 
		
<Gel2DExperiment id="ex129:Gel2DExperiment292"
	name="Analysis of leaf proteome of  Vigna mungo (T9) after MYMIV infection at 14 dpi" date="2009-05-25">
	<electrophoresisType>
		<cvParam cvRef="sepCV" accession="sep:00155" name="Two-Dimensional electrophoresis"/>
	</electrophoresisType>
	<ContactRole Contact_ref="ex129:Contact45">
        <role>
		<cvParam cvRef="sepCV" accession="sep:00035" name="principle investigator"/>
		</role>
	</ContactRole>








   



   

	
<Gel2DApplication Gel2DProtocol_ref="ex129:Gel2DProtocol292" id="ex129:Gel2DAppli292">


	
	
	<inputFirstDimension>
		


<Gel id="ex129:Gel1" name="Isoelectric focusing (IEF)" separationDimension="1">
	<gelType>IPG strip,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="Bio-rad"/>
		<model name="ReadyStripTM IPG Strip pH 3 - 10 Non-linear 17 cm strips">
			<userParam name="Model number" value="163-2009"/>
		</model>
		<userParam name="Batch number" value="Unknown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="178" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="3.3" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="0.5" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="4"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="32.3" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputFirstDimension>
	
   
	
	
	
	<inputSecondDimension>
		


<Gel id="ex129:Gel2" name="Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)" separationDimension="2">
	<gelType>slab gel,Denaturing</gelType>
	
	<GelManufactureApplication>
	
		<protocolText>
			
			

	See reference:
    Nature (1970) 227:680-685


		</protocolText>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="200" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="180" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="1.5" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="1"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="29" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputSecondDimension>
	
   

<output>
<Gel2D id="ex129:Gel2D292">

    
	
	
    
	<PHRange dimension="1">
		<Range>
			<lowerLimit><PropertyValue value="70"/></lowerLimit>
			<upperLimit><PropertyValue value="10"/></upperLimit>
		</Range>
		<rangeType><cvParam cvRef="sepCV" accession="" name=""/></rangeType>
	</PHRange>
	

    
	
	
    
	
	<MolWeightRange dimension="2">
        <Range>
            <lowerLimit><PropertyValue value="" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></lowerLimit>
            <upperLimit><PropertyValue value="7" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></upperLimit>
        </Range>
		<rangeType><cvParam cvRef="sepCV" accession="sep:00019" name="logarithmic distribution"/></rangeType>
	</MolWeightRange>
	

</Gel2D>
</output>
<ChildProtocolApplications>
<!-- TO BE DONE -->

</ChildProtocolApplications>
</Gel2DApplication>

	

<ImageAcquisitionApplication Image_ref="ex129:Image123" Material_ref="ex129:Gel2D292" ImageAcquisitionProtocol_ref="ex129:ImageAcquisitionProtocol31" id="ex129:ImageAcquisitionApplication161">
	
	<protocolText>The gel image was acquired on VersaDoc Imaging system using Qunatity One (Bio-rad) software with 1 s exposure time.</protocolText>
	
</ImageAcquisitionApplication>



	
</Gel2DExperiment>






	
		
		
	
<GelMLDataCollection>
<Image location="http://miapegeldb.expasy.org/download/329/3r9JSMok/" id="ex129:Image123" name="T9 at 14 dpi" bitDepth="8-bit (256 colors)" dimensionX="1117" dimensionY="1200">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="254" unitAccession="UO:0000242" unitName="pixels per inch" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00053" name="TIFF"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>




















<GelMLProtocolCollection>

    <ElectrophoresisProtocol
name="FirstDimension"
id="ex129:ElectrophoresisProtocol338" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex129:AddBufferAction" SubstanceMixtureProtocol_ref="ex129:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    <ElectrophoresisProtocol
name="SecondDimension"
id="ex129:ElectrophoresisProtocol339" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex129:AddBufferAction" SubstanceMixtureProtocol_ref="ex129:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    
<ImageAcquisitionProtocol id="ex129:ImageAcquisitionProtocol31">
	<equipment id="ex129:GenericEquipment45" name="VersaDoc Imaging System">
		<userParam name="type of equipment" value="VersaDoc" cvRef="sepCV" accession="sep:00096" />
		<userParam name="make" value="Bio-rad"/>
		
		<userParam name="version" value="Unknown"/>
		
	</equipment>
	<software id="ex129:GenericSoftware119" name="8.0.1" version="Unknown"/>
	<AutoCalibration id="ex129:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


</GelMLProtocolCollection>

<AuditCollection>

	<Person
id="ex129:Contact45"
name="Prof Amita Pal"
email="amita@bic.boseinst.ernet.in"
address="Division of Plant Biology,
Bose Institute, 
Kolkata 700054, 
West Bengal, India">
<affiliations
Organization_ref="ex129:Organization36"/>
</Person>

	<affiliations
Organization_ref="ex129:Organization36"
name="Bose Institute"/>

</AuditCollection>
</GelML>



