IPG strip,Denaturing slab gel,Denaturing Reagents Quantity for 900 ml of a 12.5% gel Acrylamide/PAGE 40% (w/v)= 281.25 ml PlusOne Methylenebisacrylamide 2% (w/v)= 150.3 ml Tris (1.5 M, pH 8.8)= 225 ml 10% (w/v) SDS = 9.0 ml 10% (v/v) TEMED = 1.24 ml 10% (w/v) APS = 9.0 ml Make up to 900 ml with distilled water

Default settings with 48 bit color and 16 bit grey scale scans with atleast 300 dpi [Protocol text in here] [Protocol text in here]