<?xml version="1.0" encoding="ISO-8859-1"?>
<GelML xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
		xsi:schemaLocation="http://www.psidev.info/gelml/GelML-v1_1.xsd"
		xmlns:fuge="http://fuge.sourceforge.net/fuge/1.0"
		xmlns="http://www.psidev.info/gelml/1.0"
		identifier="ExPASy:GelML:282">

<cvList>
<cv fullName="PSI sepCV" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/sep.obo" id="sepCV" version="1.0"/>
<cv fullName="Unit Ontology" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/unit.obo" id="UO" version="1.2"/>
</cvList>


 
		
<Gel2DExperiment id="ex125:Gel2DExperiment282"
	name="Mytilus edulis sperm" date="2007-12-01">
	<electrophoresisType>
		<cvParam cvRef="sepCV" accession="sep:00155" name="Two-Dimensional electrophoresis"/>
	</electrophoresisType>
	<ContactRole Contact_ref="ex125:Contact53">
        <role>
		<cvParam cvRef="sepCV" accession="sep:00035" name="principle investigator"/>
		</role>
	</ContactRole>








   



   

	
<Gel2DApplication Gel2DProtocol_ref="ex125:Gel2DProtocol282" id="ex125:Gel2DAppli282">


	
	
	<inputFirstDimension>
		


<Gel id="ex125:Gel1" name="Isoelectric focusing (IEF)" separationDimension="1">
	<gelType>IPG strip,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="GE Healthcare"/>
		<model name="Immobiline DryStrip pH 3-10 NL, 24 cm">
			<userParam name="Model number" value="17-6002-45"/>
		</model>
		<userParam name="Batch number" value="unknown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="240" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="3" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="0.5" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="4"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="29" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputFirstDimension>
	
   
	
	
	
	<inputSecondDimension>
		


<Gel id="ex125:Gel2" name="Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)" separationDimension="2">
	<gelType>slab gel,Denaturing</gelType>
	
	<GelManufactureApplication>
	
		<protocolText>
		Acrylamide/bis (30% T, 37.5:1 líquid)		
H20 mQ						
1.5 M Tris-HCL, pH 8.8				
10% SDS						
10% ammonium persulfate (fresh)			
TEMED						

		</protocolText>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="22" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
		<y value="27" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
		<z value="0.1" unitName="centimeter" unitAccession="UO:0000015" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="12.5"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="37.5" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputSecondDimension>
	
   

<output>
<Gel2D id="ex125:Gel2D282">

    
	
	
    
	<PHRange dimension="1">
		<Range>
			<lowerLimit><PropertyValue value="200"/></lowerLimit>
			<upperLimit><PropertyValue value="10"/></upperLimit>
		</Range>
		<rangeType><cvParam cvRef="sepCV" accession="" name=""/></rangeType>
	</PHRange>
	

    
	
	
    
	
	<MolWeightRange dimension="2">
        <Range>
            <lowerLimit><PropertyValue value="" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></lowerLimit>
            <upperLimit><PropertyValue value="7" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></upperLimit>
        </Range>
		<rangeType><cvParam cvRef="sepCV" accession="sep:00019" name="logarithmic distribution"/></rangeType>
	</MolWeightRange>
	

</Gel2D>
</output>
<ChildProtocolApplications>
<!-- TO BE DONE -->

</ChildProtocolApplications>
</Gel2DApplication>

	

<ImageAcquisitionApplication Image_ref="ex125:Image118" Material_ref="ex125:Gel2D282" ImageAcquisitionProtocol_ref="ex125:ImageAcquisitionProtocol44" id="ex125:ImageAcquisitionApplication155">
	
	<protocolText>We have followed manufacturer instructions</protocolText>
	
</ImageAcquisitionApplication>



	
</Gel2DExperiment>






	
		
		
	
<GelMLDataCollection>
<Image location="http://miapegeldb.expasy.org/download/402/CQBX1qCO/" id="ex125:Image118" name="Mytilus edulis sperm" bitDepth="8-bit (256 colors)" dimensionX="1000" dimensionY="1000">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="300" unitAccession="UO:0000242" unitName="pixels per inch" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00053" name="TIFF"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>




















<GelMLProtocolCollection>

    <ElectrophoresisProtocol
name="FirstDimension"
id="ex125:ElectrophoresisProtocol326" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex125:AddBufferAction" SubstanceMixtureProtocol_ref="ex125:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    <ElectrophoresisProtocol
name="SecondDimension"
id="ex125:ElectrophoresisProtocol327" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex125:AddBufferAction" SubstanceMixtureProtocol_ref="ex125:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    
<ImageAcquisitionProtocol id="ex125:ImageAcquisitionProtocol44">
	<equipment id="ex125:GenericEquipment50" name="ImageScanner (GE Healthcare)">
		<userParam name="type of equipment" value="laser scanner" cvRef="sepCV" accession="sep:00100" />
		<userParam name="make" value="GE Healthcare"/>
		
		<userParam name="version" value="Unknown"/>
		
	</equipment>
	<software id="ex125:GenericSoftware138" name="ImageMaster Platinum" version="5"/>
	<AutoCalibration id="ex125:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


</GelMLProtocolCollection>

<AuditCollection>

	<Person
id="ex125:Contact53"
name="Angel P. Diz"
email="angel.p.diz@uvigo.es"
address="Department of Biochemistry, Genetics and Immunology
Faculty of Biology
University of Vigo
Campus Universitario de Vigo s/n
36310  Vigo
Spain
Tel. +34 986813828
Population Genetics and Cytogenetics Group (XB2)
http://webs.uvigo.es/genxb2">
<affiliations
Organization_ref="ex125:Organization42"/>
</Person>

	<affiliations
Organization_ref="ex125:Organization42"
name="University of Vigo"/>

</AuditCollection>
</GelML>



