<?xml version="1.0" encoding="ISO-8859-1"?>
<GelML xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
		xsi:schemaLocation="http://www.psidev.info/gelml/GelML-v1_1.xsd"
		xmlns:fuge="http://fuge.sourceforge.net/fuge/1.0"
		xmlns="http://www.psidev.info/gelml/1.0"
		identifier="ExPASy:GelML:44">

<cvList>
<cv fullName="PSI sepCV" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/sep.obo" id="sepCV" version="1.0"/>
<cv fullName="Unit Ontology" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/unit.obo" id="UO" version="1.2"/>
</cvList>


 
		
<Gel2DExperiment id="ex12:Gel2DExperiment44"
	name="Reference map for Corynebacterium glutamicum ATCC14067, pH 4.5-5.5" date="2003-04-01">
	<electrophoresisType>
		<cvParam cvRef="sepCV" accession="sep:00155" name="Two-Dimensional electrophoresis"/>
	</electrophoresisType>
	<ContactRole Contact_ref="ex12:Contact4">
        <role>
		<cvParam cvRef="sepCV" accession="sep:00035" name="principle investigator"/>
		</role>
	</ContactRole>








   



   

	
<Gel2DApplication Gel2DProtocol_ref="ex12:Gel2DProtocol44" id="ex12:Gel2DAppli44">


	
	
	<inputFirstDimension>
		


<Gel id="ex12:Gel1" name="Isoelectric focusing (IEF)" separationDimension="1">
	<gelType>IPG strip,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="GE Healthcare Bio-Sciences Corp."/>
		<model name="Immobiline DryStrip 4.5-5.5">
			<userParam name="Model number" value="17-6001-85"/>
		</model>
		<userParam name="Batch number" value="unknown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="180" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="3" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="0.5" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="4"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="32" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputFirstDimension>
	
   
	
	
	
	<inputSecondDimension>
		


<Gel id="ex12:Gel2" name="Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)" separationDimension="2">
	<gelType>slab gel,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="GE Healthcare Bio-Sciences Corp."/>
		<model name="ExcelGel XL SDS 12-14">
			<userParam name="Model number" value="17-1236-01"/>
		</model>
		<userParam name="Batch number" value="unknown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="245" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="180" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="0.5" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="12-14"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="37.5" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputSecondDimension>
	
   

<output>
<Gel2D id="ex12:Gel2D44">

    
	
	
    
	<PHRange dimension="1">
		<Range>
			<lowerLimit><PropertyValue value="12"/></lowerLimit>
			<upperLimit><PropertyValue value="116"/></upperLimit>
		</Range>
		<rangeType><cvParam cvRef="sepCV" accession="" name=""/></rangeType>
	</PHRange>
	

    
	
	
    
	
	<MolWeightRange dimension="2">
        <Range>
            <lowerLimit><PropertyValue value="" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></lowerLimit>
            <upperLimit><PropertyValue value="7" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></upperLimit>
        </Range>
		<rangeType><cvParam cvRef="sepCV" accession="sep:00019" name="logarithmic distribution"/></rangeType>
	</MolWeightRange>
	

</Gel2D>
</output>
<ChildProtocolApplications>
<!-- TO BE DONE -->

</ChildProtocolApplications>
</Gel2DApplication>

	

<ImageAcquisitionApplication Image_ref="ex12:Image17" Material_ref="ex12:Gel2D44" ImageAcquisitionProtocol_ref="ex12:ImageAcquisitionProtocol2" id="ex12:ImageAcquisitionApplication25">
	
	<protocolText>unknown</protocolText>
	
</ImageAcquisitionApplication>



	
</Gel2DExperiment>






	
		
	
<GelMLDataCollection>
<Image location="http://world-2dpage.expasy.org/0001/html/data/gifs/yokota_0001/CORGL-ATCC14067-4.5-5.5.jpg" id="ex12:Image17" name="CORGL-ATCC14067-4.5-5.5" bitDepth="12-bit" dimensionX="2008" dimensionY="1606">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="300" unitAccession="UO:0000240" unitName="dots per inch" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00050" name="JPEG"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>




















<GelMLProtocolCollection>

    <ElectrophoresisProtocol
name="FirstDimension"
id="ex12:ElectrophoresisProtocol31" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex12:AddBufferAction" SubstanceMixtureProtocol_ref="ex12:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    <ElectrophoresisProtocol
name="SecondDimension"
id="ex12:ElectrophoresisProtocol32" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex12:AddBufferAction" SubstanceMixtureProtocol_ref="ex12:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    
<ImageAcquisitionProtocol id="ex12:ImageAcquisitionProtocol2">
	<equipment id="ex12:GenericEquipment13" name="FluorImager 595">
		<userParam name="type of equipment" value="fluorescent scanner" cvRef="sepCV" accession="sep:00100" />
		<userParam name="make" value="GE Healthcare Bio-Sciences Corp."/>
		
		<userParam name="version" value="63001545"/>
		
	</equipment>
	<software id="ex12:GenericSoftware23" name="ImageQuant" version="unknown"/>
	<AutoCalibration id="ex12:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


</GelMLProtocolCollection>

<AuditCollection>

	<Person
id="ex12:Contact4"
name="Prof. Dr. Atsushi Yokota"
email="yokota@chem.agr.hokudai.ac.jp"
address="Laboratory of Microbial Physiology
Research Faculty of Agriculture
Hokkaido University
Sapporo, Hokkaido 060-8589, Japan">
<affiliations
Organization_ref="ex12:Organization4"/>
</Person>

	<affiliations
Organization_ref="ex12:Organization4"
name="Hokkaido University"/>

</AuditCollection>
</GelML>



