<?xml version="1.0" encoding="ISO-8859-1"?>
<GelML xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
		xsi:schemaLocation="http://www.psidev.info/gelml/GelML-v1_1.xsd"
		xmlns:fuge="http://fuge.sourceforge.net/fuge/1.0"
		xmlns="http://www.psidev.info/gelml/1.0"
		identifier="ExPASy:GelML:272">

<cvList>
<cv fullName="PSI sepCV" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/sep.obo" id="sepCV" version="1.0"/>
<cv fullName="Unit Ontology" URI="https://psidev.svn.sourceforge.net/svnroot/psidev/psi/sepcv/trunk/unit.obo" id="UO" version="1.2"/>
</cvList>


 
		
<Gel2DExperiment id="ex118:Gel2DExperiment272"
	name="f1INIp310g63361" date="2008-07-15">
	<electrophoresisType>
		<cvParam cvRef="sepCV" accession="sep:00155" name="Two-Dimensional electrophoresis"/>
	</electrophoresisType>
	<ContactRole Contact_ref="ex118:Contact49">
        <role>
		<cvParam cvRef="sepCV" accession="sep:00035" name="principle investigator"/>
		</role>
	</ContactRole>








   



   

	
<Gel2DApplication Gel2DProtocol_ref="ex118:Gel2DProtocol272" id="ex118:Gel2DAppli272">


	
	
	<inputFirstDimension>
		


<Gel id="ex118:Gel1" name="Isoelectric focusing (IEF)" separationDimension="1">
	<gelType>IPG strip,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="GE-healtcare"/>
		<model name="ImmobilineTM DryStrip pH 3-10 linear 11 cm strips ">
			<userParam name="Model number" value="18-1016-61"/>
		</model>
		<userParam name="Batch number" value="63361-63362"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="108" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="3" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="0.5" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="4"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="32" crossLinker="1">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputFirstDimension>
	
   
	
	
	
	<inputSecondDimension>
		


<Gel id="ex118:Gel2" name="Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)" separationDimension="2">
	<gelType>slab gel,Denaturing</gelType>
	
	<GelManufactureApplication>
		
		<make name="Bio-rad"/>
		<model name="criterion XT bis-tris gel 10%">
			<userParam name="Model number" value="3450115"/>
		</model>
		<userParam name="Batch number" value="unknown"/>
		
	</GelManufactureApplication>
	<Dimensions>
		
		
		<x value="133" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<y value="87" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
		<z value="1" unitName="millimeter" unitAccession="UO:0000082" unitCvRef="UO"/>
	</Dimensions>
	
	<percentAcrylamide>
		<AtomicValue><PropertyValue value="10"/></AtomicValue>
	</percentAcrylamide>
	<AcrylamideToCrossLinker acrylamide="10" crossLinker="5">
		<crossLinkerType>
			<cvParam cvRef="sepCV" accession="sep:00190" name="Bisacrylamide"/>
		</crossLinkerType>
	</AcrylamideToCrossLinker>	
</Gel>

	</inputSecondDimension>
	
   

<output>
<Gel2D id="ex118:Gel2D272">

    
	
	
    
	<PHRange dimension="1">
		<Range>
			<lowerLimit><PropertyValue value="10"/></lowerLimit>
			<upperLimit><PropertyValue value="250"/></upperLimit>
		</Range>
		<rangeType><cvParam cvRef="sepCV" accession="" name=""/></rangeType>
	</PHRange>
	

    
	
	
    
	
	<MolWeightRange dimension="2">
        <Range>
            <lowerLimit><PropertyValue value="" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></lowerLimit>
            <upperLimit><PropertyValue value="7" unitName="kilodaltons" unitCvRef="UO" unitAccession="UO:0000222"/></upperLimit>
        </Range>
		<rangeType><cvParam cvRef="sepCV" accession="sep:00019" name="logarithmic distribution"/></rangeType>
	</MolWeightRange>
	

</Gel2D>
</output>
<ChildProtocolApplications>
<!-- TO BE DONE -->

</ChildProtocolApplications>
</Gel2DApplication>

	

<ImageAcquisitionApplication Image_ref="ex118:Image110" Material_ref="ex118:Gel2D272" ImageAcquisitionProtocol_ref="ex118:ImageAcquisitionProtocol37" id="ex118:ImageAcquisitionApplication147">
	
	<protocolText>exposure time settings
gel1
cy2 0.7
cy3 0.2
cy5 0.35
gel2
0.8
0.2
0.3</protocolText>
	
</ImageAcquisitionApplication>



	
</Gel2DExperiment>






	
		
		
	
<GelMLDataCollection>
<Image location="http://miapegeldb.expasy.org/download/356/9o59KOxP/" id="ex118:Image110" name="f1inip310g63361" bitDepth="24-bit (TrueColor)" dimensionX="2192" dimensionY="1471">
<resolution>
<AtomicValue>
    
        
    
	<PropertyValue value="40" unitAccession="UO:0000239" unitName="micrometers per pixel" unitCvRef="UO"/>
</AtomicValue>
</resolution>
<fileFormat>
	
		
	
	<cvParam cvRef="sepCV" accession="sep:00050" name="JPEG"/>
</fileFormat>
<userParam name="Standard image orientation" value="Yes"/>
</Image>
</GelMLDataCollection>




















<GelMLProtocolCollection>

    <ElectrophoresisProtocol
name="FirstDimension"
id="ex118:ElectrophoresisProtocol310" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex118:AddBufferAction" SubstanceMixtureProtocol_ref="ex118:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    <ElectrophoresisProtocol
name="SecondDimension"
id="ex118:ElectrophoresisProtocol311" >
<protocolText>[Protocol text in here]</protocolText>
<AddBufferAction id="ex118:AddBufferAction" SubstanceMixtureProtocol_ref="ex118:SubstanceMixtureProtocol0"/>
</ElectrophoresisProtocol>

    
<ImageAcquisitionProtocol id="ex118:ImageAcquisitionProtocol37">
	<equipment id="ex118:GenericEquipment48" name="Ettan Dige Imager">
		<userParam name="type of equipment" value="fluorescent scanner" cvRef="sepCV" accession="sep:00100" />
		<userParam name="make" value="GE-healtcare"/>
		
		<userParam name="version" value="11-0036-58"/>
		
	</equipment>
	<software id="ex118:GenericSoftware128" name="EDI software" version="1.0"/>
	<AutoCalibration id="ex118:AutoCalib0">
		<BooleanValue value="false"/>
	</AutoCalibration>
	
	
</ImageAcquisitionProtocol>


</GelMLProtocolCollection>

<AuditCollection>

	<Person
id="ex118:Contact49"
name="Dr Jonathan Ewbank"
email="ewbank@ciml.univ-mrs.fr"
address="Research group of Innate immunity in C. elegans

Case 906
163 avenue de luminy
13288 Marseille Cedex 9
France">
<affiliations
Organization_ref="ex118:Organization39"/>
</Person>

	<affiliations
Organization_ref="ex118:Organization39"
name="Centre d'Immunologie de Marseille Luminy"/>

</AuditCollection>
</GelML>



