IPG strip,Denaturing slab gel,Denaturing amount for six gels: 30 % (w/v) Acrylamide/Bisacrylamide (188 ml), 1.5 M Tris-Cl pH 8.8 (113 ml), Double distilled water (140 ml), 10% (w/v) SDS (4.5 ml), 10% (w/v) ammonium persulfate (4.5 ml), 10% (w/v) TEMED (620 µl)

The gel image was acquired in 16 bit grayscale with a resolution of 300 dpi and was saved in tif format. No filters or image processing tools were used during aquisition. A Kodak Step Tablet no. 2 (GE Healthcare Bio-Sciences Corp.) was scanned using the same parameters. An intensity calibration was performed within the image analysis software Image Master 2D Platinum (GE Healthcare Bio-Sciences Corp.) as described in the manual of the software. [Protocol text in here] [Protocol text in here]