IPG strip,Denaturing slab gel,Denaturing Separation gel (12 % polyacrylamide) (2 gels) 26.8 ml ddH2O 20 ml 1.5 M TrisHCl pH 8.8 (Roth, Germany) 32 ml Acrylamide / bisacrylamide (37.5:1) (Serva, Germany) 800 μl SDS 10 % (Serva, Germany) 400 μl Ammonium persulfate 10 % (Merck, Germany) 40 μl N,N,N’,N’-Tetramethylethylendiamine (TEMED) (Roth, Germany) Stacking gel (4 % polyacrylamide) (2 gels) 6.1 ml ddH2O 2.5 ml 0.5 M TrisHCl pH 6.8 (Roth, Germany) 1.3 ml Acrylamide / bisacrylamide (37.5:1) (Serva, Germany) 100 μl SDS 10 % (Serva, Germany) 50 μl Ammonium persulfate 10 % (Merck, Germany) 10 μl N,N,N’,N’-Tetramethylethylendiamine (TEMED) (Roth, Germany)

According to manufacturer [Protocol text in here] [Protocol text in here]