IPG strip,Denaturing
slab gel,Denaturing
Separation gel (12 % polyacrylamide) (2 gels)
26.8 ml ddH2O
20 ml 1.5 M TrisHCl pH 8.8 (Roth, Germany)
32 ml Acrylamide / bisacrylamide (37.5:1) (Serva, Germany)
800 μl SDS 10 % (Serva, Germany)
400 μl Ammonium persulfate 10 % (Merck, Germany)
40 μl N,N,N’,N’-Tetramethylethylendiamine (TEMED) (Roth, Germany)
Stacking gel (4 % polyacrylamide) (2 gels)
6.1 ml ddH2O
2.5 ml 0.5 M TrisHCl pH 6.8 (Roth, Germany)
1.3 ml Acrylamide / bisacrylamide (37.5:1) (Serva, Germany)
100 μl SDS 10 % (Serva, Germany)
50 μl Ammonium persulfate 10 % (Merck, Germany)
10 μl N,N,N’,N’-Tetramethylethylendiamine (TEMED) (Roth, Germany)
According to manufacturer
[Protocol text in here]
[Protocol text in here]