IPG strip,Denaturing slab gel,Denaturing H2O 33 mL, 30%acrylamide mix 40ml, 1.5M Tris (pH8.8) 25ml, 10% SDS 1ml, 10% ammonium persulfate 1ml, and 0.04ml of TEMED

excitation wavelength of 633nm and without an emission filter PMT 600 scan time 15 min [Protocol text in here] [Protocol text in here]